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This Concept Map, created with IHMC CmapTools, has information related to: LAB 12 - ISOLATION AND IDENTIFICATION OF ENTEROBACTERIACEAE AND PSEUDOMONAS, PART 1, cause opportunistic infections wound infections, opportunistic pathogens medically-important genera Escherichia, XLD agar Selective for Gram-negative enteric bacteria, especially intestinal pathogens such as Salmonella and Shigella. it detects fermentation of the sugars lactose and sucrose, dercarboxylation of the amino acid L-lysine, and the production of hydrogen sulfide. examples Shigella, Pseudomonas identification pigment production and fluorescence, Pseudomonas causes opportunistic infections septicemia, oxidase test (Taxo-N® disc ) results P.aeruginosa and most other non-fermentative Gram-negative rods are oxidase-positive; most Enterobacteriaceae are oxidase-negative., the Enterobacteriaceae medically-important Enterobacteriaceae opportunistic pathogens, non-fermentative Gram-negative bacilli examples Acinetobacter, Aeromonas, Alcaligenes, Eikenella, Flavobacterium, and Moraxella., gastrointestinal (gi) pathogens medically-important genera Shigella, selected Gram-negative bacilli of medical importance examples non-fermentative Gram-negative bacilli, oxidase test results Almost all Enterobacteriaceae are oxidase-negative, cause opportunistic infections urinary tract infections, Run an Gibson oxidase test on your unknown. oxidase-positive Inoculate a Cetrimide agar plate and an Enteropluri-Test. Incubate at 37C., Pseudomonas isolation Cetrimide agar: selective for Pseudomonas aeruginosa and stimulates P. aeruginosa to produce its characteristic pigment as well as fluorescent products., Shigella appearance red colonies without black centers, cause opportunistic infections septicemia, Pseudomonas identification odor, the Enterobacteriaceae medically-important Enterobacteriaceae gastrointestinal (gi) pathogens, Escherichia coli appearance The colonies and confluent growth to appear bright pink to red with a pink precipitate (cloudiness) appearing in the agar surrounding the growth, Enterobacteriaceae isolation on XLD Agar XLD agar Selective for Gram-negative enteric bacteria, especially intestinal pathogens such as Salmonella and Shigella. it detects fermentation of the sugars lactose and sucrose, dercarboxylation of the amino acid L-lysine, and the production of hydrogen sulfide.
